Action of Clostridium perfringens alpha toxin.
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چکیده
منابع مشابه
Nutritional Requirements of Clostridium Perfringens Pb6k for Alpha Toxin Production
A potent toxin was produced by a strain of Clostridium perfringens PB6K in a chemically defined medium consisting of 19 amino acids, fructose, vitamins and minerals. A large amount of L-arginine (10 mg/ml or higher concentration) was required for toxin production. An excess of L-cystine (0.1 mg/ml or higher) reduced the yield of toxin seriously. Zinc and manganese, though ineffective for growth...
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Clostridium perfringens alpha-toxin is a key mediator of gas gangrene, which is a life-threatening infection that manifests as fever, pain, edema, myonecrosis, and gas production. Alpha-toxin possesses phospholipase C and sphingomyelinase activities. The toxin is composed of an N-terminal domain (1-250 aa, N-domain), which is the catalytic site, and a C-terminal domain (251-370 aa, C-domain), w...
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Iota toxin is produced by Clostridium perfringens type E. This toxin causes antibiotic-associated enterotoxemia in lambs and calves. Iota toxin is a binary toxin that has two components including Ia (the enzyme component) and Ib (the binding component). Ib binds to the surface receptor of target cells and translocate Ia into the cytosol of cells. The aim of this study was to clone toxigenic epi...
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Clostridium perfringens produces alpha-toxin, which is an important virulence factor in gas gangrene [1-3]. Alpha-toxin is hemolytic, dermonecrotic, and lethal. Furthermore, it has phospholipase C (PLC) and sphingomyelinase (SMase) activities [13]. The toxin has been shown to damage the membranes of various mammalian cells [1-3] as well as artificial membranes [4]. The structure of alpha-toxin ...
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Described is a rapid direct sandwich format electrochemiluminescence assay for identifying and assaying Clostridium perfringens alpha toxin. Biotinylated antibodies to C. perfringens alpha toxin bound to streptavidin paramagnetic beads specifically immunoadsorbed soluble sample alpha toxin which subsequently selectively immunoadsorbed ruthenium (Ru)-labeled detection antibodies. The ruthenium c...
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ژورنال
عنوان ژورنال: Nippon Saikingaku Zasshi
سال: 1994
ISSN: 1882-4110,0021-4930
DOI: 10.3412/jsb.49.719